General method for amplifying regions of very high G + C content
نویسندگان
چکیده
منابع مشابه
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Nieders~chsisches Institut ffir Peptid-Forschung GmbH, D-30625 Hannover, Germany PCR amplification of sequences from genomic DNA often yields multiple nonspecific bands, especially if the amplified regions are rich in guanosine and cytosine. G/C-rich sequences can cause hairpin loop structures that potentially give rise to problems for in vitro DNA amplification. In addition to this, G/Crich pr...
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Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.
Two versions of denaturation fingerprinting (dnF2R and dnF1R are described for detecting mutations. DnF2R is a sensitive screening method in which fingerprints are generated by performing denaturing gel electrophoresis on bidirectional "cycle-sequencing" reactions with each of two dideoxy terminators, e.g. ddATP and ddCTP. When the fingerprints generated by ddATP and ddCTP are combined, all seq...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.12.2953